A Yeast Surface Display System for Streamlined Phage Endolysin Production and Use

Abstract

one possible substitute for traditional antibiotics is bacteriophage endolysin; nevertheless,
the comprehensive use of this therapeutic protein may be jeopardized by the inherent constraints of
the bacterial expression system. We used a yeast surface display system as a unique endolysin
expression platform in order to get around these restrictions. Saccharomyces cerevisiae produced and
surface-displayed endolysin LysSA11 from staphylococcal phage SA11 to demonstrate adequate
antibacterial activity against Staphylococcus aureus. We demonstrated that direct treatment of yeast
cells expressing LysSA11 could achieve a 5-log reduction of viable Staphylococcus aureus in 3 hours
without the need for any protein extraction or purification techniques. Additionally, after 14 days of
storage in a chilled environment, the surface-displayed LysSA11 demonstrated greater stability than
the soluble form of purified LysSA11. We propose that the yeast surface display system is a simple,
reliable, and effective platform for endolysin synthesis and antibacterial uses.