A Yeast Surface Display System for Streamlined Phage Endolysin Production and Use

Abstract

One possible substitute for traditional antibiotics is bacteriophage endolysin; nevertheless, the
comprehensive use of this therapeutic protein may be jeopardized by the inherent constraints of the bacterial
expression system. We used a yeast surface display system as a unique endolysin expression platform in
order to get around these restrictions. Saccharomyces cerevisiae produced and surface-displayed endolysin
LysSA11 from staphylococcal phage SA11 to demonstrate adequate antibacterial activity against
Staphylococcus aureus. We demonstrated that direct treatment of yeast cells expressing LysSA11 could
achieve a 5-log reduction of viable Staphylococcus aureus in 3 hours without the need for any protein
extraction or purification techniques. Additionally, after 14 days of storage in a chilled environment, the
surface-displayed LysSA11 demonstrated greater stability than the soluble form of purified LysSA11. We
propose that the yeast surface display system is a simple, reliable, and effective platform for endolysin
synthesis and antibacterial uses